Blood Cancer Standards

A rapid shift to NGS  Myeloid Cancer Image

The molecular analysis of myeloid cancers is swiftly moving from expensive, time-consuming methods like FISH and qPCR to highly multiplexed next-generation sequencing (NGS) assays. There are now several FDA-approved NGS-based diagnostic tests for profiling hematologic malignancies, with more in development, thanks to NGS’ ability to interrogate a broad panel of targets at high resolution.

Overcoming validation challenges

But this is also why it can be so challenging to validate these assays. Because there is such a vast diversity of mutations across many genes that are relevant for myeloid cancers, it can be a monumental task to source sufficient quantities of remnant patient specimens and cell lines bearing clinically relevant variants.

Blood cancer profiling demands purpose-built QC materials

To both accelerate and lower the cost of validating these highly multiplexed assays, we have produced the first highly multiplexed biosynthetic reference material purpose-built for NGS-based tests for hematologic malignancies. This expert-designed material includes 23 DNA mutations and nine RNA fusions common to the latest NGS-based myeloid assays.


KEY GENES INCLUDED IN SERASEQ MYELOID DNA & FUSION RNA

ABL1

BRAF

CEBPA

IDH1

ASXL1

CBL

FLT3

JAK2

BCR-ABL

EVT6-ABL1

PCM1-JAK2

PML-RARα

Seraseq myeloid reference materials are intended to be compatible with commonly available myeloid assays such as those listed below.

  • Thermo Fisher Oncomine® assay
  • Archer® VariantPlex® and FusionPlex®
  • Illumina TruSight® Myeloid Sequencing Panel
  • QIAGEN QIAseq® Targeted DNA & RNA Scan Panels

Note that there may be differences in observed allele frequencies across variants due to specific assay characteristics. Please consult with specific manufacturer protocols and specifications.

Click here to view the full variant list

Powerful technology for patient-like material

Our Seraseq biosynthetic technology allows us to build highly multiplexed reference materials that produce data virtually identical to a patient sample with the same variant. These truth-set materials feature allele frequencies precisely quantitated by digital PCR assays. Save time and money by cutting your specimen search time while getting a wealth of data around assay performance in a single run.

Product Listing Material Number
Seraseq™ Myeloid Fusion RNA Mix 0710-0407
Seraseq™ Myeloid Mutation DNA Mix 0710-0408