Frequently Asked Questions
What type of material comprises the AccuPlex™ SARS-CoV-2 products?
AccuPlex™ SARS-CoV-2 products are recombinant RNA based material developed using proprietary AccuPlex technology, which is based on an Alphavirus vector system whereby the genes for structural proteins in the genomic RNA are replaced with target sequences from SARS-CoV-2. Please see the SARS-CoV-2 Molecular Product Sheet for more information.
Are the AccuPlex SARS-CoV-2 quality solutions products infectious?
Product is non-infectious, non-replicative recombinant virus that has been heat-treated (56°C for 40 minutes). However, use Centers for Disease Control (CDC) recommended universal precautions when handling AccuPlex SARS-CoV-2 reference material.
What is the difference between the Verification Panel and the Reference Material?
AccuPlex SARS-CoV-2 Verification Panel is a robust solution for initial assay installation & performance verification as it has 3 different concentration levels to assess performance across the entire assay dynamic range. It is ideally used in conjunction with the AccuPlex SARS-CoV-2 Reference Material Kit for daily and long-term monitoring & performance evaluation. Both products could be used hand-in-hand. We offer different versions of these products offering different SARS-CoV-2 sequence coverage. Please see the SARS-CoV-2 Molecular Product Sheet for more information.
Are these molecular quality solutions compatible with my SARS-CoV-2 assay?
Material is compatible with assays targeting CDC and WHO consensus sequences. Version 2 of the product (0505-0133) additionally includes S gene targets. The latest release (0505-0168, 0505-0159) includes the full SARS-CoV-2 viral genome. AccuPlex products can be customized for sequences of interest to meet unique assay design requirements.
Do I need to perform extraction before using the AccuPlex SARS-CoV-2 product?
Yes, this material must go through an extraction process, prior to detection by PCR. Process the product according to the instructions for unknown samples provided by the test kit or the laboratory’s standard operating procedures. Note: if your protocol calls for a separate heat treatment of the patient sample prior to processing, the AccuPlex SARS CoV-2 material does not require this step. It has already undergone a heat treatment process.
What is the targeted concentration of the AccuPlex SARS-CoV-2 material?
Reference Kit: Targeted to 5,000 copies/mL using digital PCR
Verification Panel: Member 1: 100,000 cp/mL, Member 2: 10,000 cp/mL, Member 3: 1,000 cp/mL, Member 4: Negative
Please see the SARS-CoV-2 Molecular Product Sheet for more information.
How many tests can I get from a Reference Material kit?
The number of tests varies based on assay input volume and LOD.
What should I use for diluent?
Please refer to the test method package insert for specific information; however, we would recommend universal/viral transport media. If UTM/VTM is not available, PBS can be used for dilutions prior to performing the assay. Note: while PBS can be used at time of testing, storage in PBS is not recommended.
What is the shelf life of the AccuPlex SARS-CoV-2 material?
2 years from the date of manufacture
Are materials available for serological SARS-CoV-2 assays?
Yes, please learn more about our ACCURUN SARS-CoV-2 Serology Quality Solutions to ensure antibody testing accuracy and performance.