BacTrace® Anti-E. coli O45 Antibody

  • Size

    0.1 mg

  • List Price

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  • Unit


  • Inventory Status

    In Stock


BacTrace® affinity purified polyclonal antibody to E. coli O45 is isolated from a serum pool of goats immunized with heat-killed whole cells of E. coli serogroup O45. Certain strains of the E. coli serogroup O45 are considered to be STECs (shiga toxin-producing E. coli).This antibody can be used in a variety of immunoselection and immunodetection applications.

Product Highlights:

  • High specificity to E. coli O45, minimal cross-reactivity to other E. coli serogroups
  • Polyclonal antibody yields higher sensitivity than monoclonal antibodies
  • Standardized lots provide consistent performance


  • Immunogen: Heat-killed whole cells of E. coli serotype O45
  • Purification: Affinity purification
  • Specificity: E. coli O45
  • Host: Goat
  • Form: Whole IgG
  • Label: None
  • Physical State: Lyophilized
  • Stabilizers/Preservatives: None
  • Suggested Working Dilutions: Capture Antibody: 10 μg/mL, Primary Antibody: 1 – 2 μg/mL
  • Stability: 1 year from date of receipt
  • Storage: 2 - 8°C

The plot depicts OD values from a TMB-detected HRP capture ELISA. SeraCare's KPL heat-killed positive controls from various STEC serogroups and S. typhimurium (1:100 dilution, ~107 CFU/mL) were used as analytes. Unlabeled Anti-E. coli O45 antibody was used as the capture antibody, and HRP-labeled conjugate was used as the reporter. All other assay components were SeraCare catalog products. The figure below demonstrates the high specificity of BacTrace Anti-E.coli O45 in an ELISA. Cross-reactivity is minimal with other E. coli groups and non-E. coli bacteria.