Whole transcriptome RNA-sequencing (RNA-Seq) has emerged as one of the most effective methods for detection of genomic rearrangements in cancer. Enrichment for poly(A) + RNA as part of library preparation is a standard method to select for mRNAs, but ribosomal RNA depletion and exon capture methods are also used. Seraseq® Fusion RNA Mix v4 was developed for targeted NGS panels and performed sub-optimally on RNA-Seq.

Optimizing Performance of Whole Transcriptome RNA-Seq Reference Materials

Download this poster from AACR to learn more on how we tested whether increasing the length of the poly-A tail would improve the efficiency of binding to an oligo dT column and improve performance in total RNA-Seq NGS assays and evaluated whether increasing the prevalence of the fusion RNA transcripts relative to the total RNA may improve performance on RNA-Seq methods. that use poly(A) + selection. 

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