Controls & Reference Materials

Seraseq® ctDNA Mutation Mix v2 AF0.125%

Material Number 0710-0143
Size 1 x 25 µL
Inventory Status In Stock
Unit EA

To overcome the lack of patient-like reference materials for liquid biopsy, including drawbacks of existing methodologies such as sonication, SeraCare has developed a breakthrough technology that produces the most patient-like materials compared to native ctDNA. The Seraseq ctDNA Mutation Mix v2 is a highly multiplexed product for NGS-based ctDNA assays targeting cancer-relevant somatic mutations.

This specific ctDNA Mutation Mix v2 product has an allele frequency of 0.125%.

  • Size distribution, library yield and complexity consistent with native ctDNA
  • Single sample with 40 clinically-relevant mutations across 28 genes, all at the same specified allele frequency
  • Offered as a purified DNA mixture for ease of use during assay development
  • Mutation targets precisely quantitated with digital PCR
  • Single well-characterized GM24385 human genomic DNA as background wild-type material
  • Manufactured in GMP-compliant and ISO 13485-certified facilities

Seraseq ctDNA v2 Reference Materials

Doc type
Product Sheet
Lot #
--

Seraseq ctDNA Mutation Mix v2 AF0.125%

Doc type
Product Report
Lot #
10321440

Seraseq ctDNA Mutation Mix v2

Doc type
Package Insert

Seraseq ctDNA Mutation Mix v2 AF0.125%

Doc type
Product Report
Lot #
10269364
Product Specifications
# of variants 40
Allele Frequency 0.125%
Fragment Size ~170bp
Concentration 10 ng/µL
Fill Size 25 µL
Total DNA 250 ng
Buffer Tris/EDTA (TE)

 

NGS-Based Assay Compatibility Chart
Archer® Reveal ctDNA™ 28 Kit Oncomine® Pan-
Cancer Cell-Free Assay
Oncomine Lung
cfDNA Assay
Oncomine
Colon cfDNA
UltraSEEK™
Colon Panel
UltraSEEK
Lung Panel 
AKT1 AKT1 ALK AKT1 BRAF BRAF 
ALK ALK BRAF BRAF EGFR EGFR 
BRAF APC EGFR EGFR KRAS ERBB2 
CTNNB1 BRAF ERBB2 ERBB2 NRAS KRAS
EGFR CTNNB1 KRAS KRAS PIK3CA PIK3CA 
ERBB2 EGFR NRAS NRAS    
IDH1 ERBB2 PIK3CA PIK3CA    
KIT FGFR3 ROS1 SMAD4     
KRAS FLT3 TP53 TP53     
NRAS GNA11        
PDGFRA GNAQ        
PIK3CA GNAS        
RET IDH1        
ROS1 KIT        
SMAD4 KRAS        
TP53 NRAS        
  PDGFRA        
  PIK3CA        
  PTEN        
  TP53        
  ROS1        
  RET        
Archer® is a registered trademark, and Reveal ctDNA™ is a trademark of ArcherDx, Inc.
Oncomine® is a registered trademark of Thermo Fisher Scientific.
UltraSEEK™ is a trademark of Agena Bioscience, Inc. 

Find more data and in-depth technical information by reading this technical product report.