Liquid Biopsy
Seraseq® ctDNA Complete™ Reference Material AF5%
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To further accelerate the validation and clinical application of liquid biopsy-based ccfDNA targeted NGS assays, SeraCare has developed the Seraseq ctDNA Complete that expands coverage of all relevant variant types (SNVs, INDELs, CNVs, and gene fusions) in a single, highly multiplexed reference sample. These new products are offered in either a purified ctDNA format or a plasma-like matrix format, all precisely quantitated by digital PCR against a single well-characterized genomic background (GM24385), and orthogonally validated with NGS technology.
This specific ctDNA Complete product has an allele frequency of 5%.
- 25 unique multiplexed variants in 16 genes, covering 12 SNVs, 7 INDELs, 3 CNVs, and 3 SVs, in purified and plasma-like matrix formats
- 6 different allele frequencies (AF) – WT, 0.1%, 0.5%, 1%, 2.5% and 5%
- Allows end-to-end evaluation of assay performance across the entire workflow, including pre-analytic extraction steps
- Variants quantitated with digital PCR and orthogonally validated by NGS
- Blends with well-characterized GM24385 human genomic DNA as background ‘wild-type’ material
- Manufactured within cGMP compliant, ISO 9001 and ISO 13485 certified facilities
Seraseq ctDNA Complete Reference Materials Package Insert
Seraseq ctDNA Complete Reference Material AF5%
Seraseq ctDNA Complete Reference Material AF5%
Seraseq ctDNA Complete Reference Material AF5%
Technical Spreadhseet for Seraseq ctDNA Complete Mutation Mix and Reference Material
Seraseq ctDNA Complete
Seraseq ctDNA Complete Reference Material AF5%, AF2.5%, AF1%, AF0.5%, AF0.1%, WT (Wild-Type)
Seraseq ctDNA Complete Reference Material Non-Requirement Letter
Seraseq ctDNA Complete Reference Material AF5%
Seraseq ctDNA Complete Reference Material AF5%
Seraseq ctDNA Complete Reference Material Technical Spreadsheet
Product Specifications | |
# of Variants | 25 |
Allele Frequency | 5% |
Fragment Size | ~170bp average |
Concentration | 25 ng/mL |
Volume | 5 mL |
Amount | 125 ng (single vial) |
Matrix | SeraCon™ Matribase (human plasma-like) |
NGS-Based Assay Compatibility Chart | |||||
Archer® Reveal ctDNA™ 28 Kit | Oncomine® Pan- Cancer Cell-Free Assay |
Oncomine Lung cfDNA Assay |
Oncomine Colon cfDNA |
UltraSEEK™ Colon Panel |
UltraSEEK Lung Panel |
AKT1 | AKT1 | ALK | AKT1 | BRAF | BRAF |
ALK | ALK | BRAF | BRAF | EGFR | EGFR |
BRAF | BRAF | EGFR | EGFR | KRAS | ERBB2 |
EGFR | EGFR | ERBB2 | ERBB2 | NRAS | KRAS |
ERBB2 | ERBB2 | KRAS | KRAS | PIK3CA | PIK3CA |
KIT | KIT | MET | NRAS | ||
KRAS | KRAS | NRAS | PIK3CA | ||
MET | MET | PIK3CA | |||
NRAS | MYC | ROS1 | |||
PIK3CA | NRAS | ||||
RET | PIK3CA | ||||
Archer® is a registered trademark, and Reveal ctDNA™ is a trademark of ArcherDx, Inc. Oncomine® is a registered trademark of Thermo Fisher Scientific. UltraSEEK™ is a trademark of Agena Bioscience, Inc. |
Find more data and in-depth technical information by reading this Technical Product Report.
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