Liquid Biopsy
Seraseq® ctDNA Complete™ Reference Material AF0.5%
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To further accelerate the validation and clinical application of liquid biopsy-based ccfDNA targeted NGS assays, SeraCare has developed the Seraseq ctDNA Complete that expands coverage of all relevant variant types (SNVs, INDELs, CNVs, and gene fusions) in a single, highly multiplexed reference sample. These new products are offered in either a purified ctDNA format or a plasma-like matrix format, all precisely quantitated by digital PCR against a single well-characterized genomic background (GM24385), and orthogonally validated with NGS technology.
This specific ctDNA Complete product has an allele frequency of 0.5%.
- 25 unique multiplexed variants in 16 genes, covering 12 SNVs, 7 INDELs, 3 CNVs, and 3 SVs, in purified and plasma-like matrix formats
- 6 different allele frequencies (AF) – WT, 0.1%, 0.5%, 1%, 2.5% and 5%
- Allows end-to-end evaluation of assay performance across the entire workflow, including pre-analytic extraction steps
- Variants quantitated with digital PCR and orthogonally validated by NGS
- Blends with well-characterized GM24385 human genomic DNA as background ‘wild-type’ material
- Manufactured within cGMP compliant, ISO 9001 and ISO 13485 certified facilities
Seraseq ctDNA Complete Reference Materials Package Insert
Technical Spreadhseet for Seraseq ctDNA Complete Mutation Mix and Reference Material
Seraseq ctDNA Complete Reference Material AF0.5%
Seraseq ctDNA Complete Reference Material AF5%, AF2.5%, AF1%, AF0.5%, AF0.1%, WT (Wild-Type)
Seraseq ctDNA Complete Reference Material Non-Requirement Letter
Seraseq ctDNA Complete Reference Material AF0.5%
Seraseq ctDNA Complete
Seraseq ctDNA Complete Reference Material AF0.5%
Seraseq ctDNA Complete Reference Material AF0.5%
Seraseq ctDNA Complete Reference Material Technical Spreadsheet
Product Specifications | |
# of Variants | 25 |
Allele Frequency | 0.5% |
Fragment Size | ~170bp average |
Concentration | 25 ng/mL |
Volume | 5 mL |
Amount | 125 ng (single vial) |
Matrix | SeraCon™ Matribase (human plasma-like) |
NGS-Based Assay Compatibility Chart | |||||
Archer® Reveal ctDNA™ 28 Kit | Oncomine® Pan- Cancer Cell-Free Assay |
Oncomine Lung cfDNA Assay |
Oncomine Colon cfDNA |
UltraSEEK™ Colon Panel |
UltraSEEK Lung Panel |
AKT1 | AKT1 | ALK | AKT1 | BRAF | BRAF |
ALK | ALK | BRAF | BRAF | EGFR | EGFR |
BRAF | BRAF | EGFR | EGFR | KRAS | ERBB2 |
EGFR | EGFR | ERBB2 | ERBB2 | NRAS | KRAS |
ERBB2 | ERBB2 | KRAS | KRAS | PIK3CA | PIK3CA |
KIT | KIT | MET | NRAS | ||
KRAS | KRAS | NRAS | PIK3CA | ||
MET | MET | PIK3CA | |||
NRAS | MYC | ROS1 | |||
PIK3CA | NRAS | ||||
RET | PIK3CA | ||||
Archer® is a registered trademark, and Reveal ctDNA™ is a trademark of ArcherDx, Inc. Oncomine® is a registered trademark of Thermo Fisher Scientific. UltraSEEK™ is a trademark of Agena Bioscience, Inc. |
Find more data and in-depth technical information by reading this Technical Product Report.
Liquid Biopsy FAQs
Review the common questions we receive from our customers and the responses we provide.