TMB 1-Component Membrane Peroxidase Substrate

Size Material # List Price Qty  
200 mL 5420-0027 $102.00 Add to Cart
1 L 5420-0028 $367.00 Add to Cart
100 mL 5420-0029 $63.00 Add to Cart


Ready-to-use TMB Membrane Peroxidase Substrate reacts with peroxidase conjugates that have bound to proteins that have been immobilized on membranes following Western or dot blotting. The substrate provides sensitivity in the picogram range with minimal background. It is the most sensitive chromogenic substrate for peroxidase detection. It develops a deep blue color at the reaction site in the presence of peroxidase-labeled conjugates. Recommended for membrane assays and dot blots. 1-component format of 3, 3', 5, 5' - Tetramethylbenzidine, no reagent preparation, hence, very convenient.

2 x 100 mL 1-Component TMB Membrane Peroxidase Substrate

Product Highlights:

  • Most sensitive HRP colorimetric substrate for blotting
  • Minimal background
  • Distinct blue reaction product


  • Detection Method: Colorimetric Western or dot blotting
  • Sensitivity: Picogram
  • Required Enzyme: HRP
  • Stability: Minimum 1 year from date of receipt
  • Storage: 2 - 8°C