Protein Detector™ TMB Western Blotting Kit
Protein Detector Western Blot Kits are designed for the colorimetric detection of proteins immobilized on membranes through either electrophoresis or dot blotting. Using the Protein Detector TMB Western Blot Kit, the combination of a highly specific, stable liquid conjugate with a sensitive chromogenic substrate allows rapid and accurate identification of samples.
All solutions required for blocking and washing the membrane and for diluting antibodies are provided. For added flexibility, affinity purified anti-mouse and anti-rabbit secondary antibodies conjugated with horseradish peroxidase are provided in stabilized, liquid form for quick dilution. TMB Membrane Peroxidase Substrate is provided as a convenient ready-to-use solution, which forms an insoluble blue precipitate when combined with the HRP-labeled antibody.
2 x 120 mL Detector Block Solution, 10 g Detector Block Powder, 3 x 100 mL Wash Solution Concentrate, 75 µL peroxidase-labeled Anti-Rabbit IgG (H+L) and peroxidase-labeled Anti-Mouse IgG (H+L), 1 x 200 mL TMB Membrane Peroxidase Substrate
- Most sensitive colorimetric HRP substrate for blotting
- High signal-to-noise, minimal background
- Anti-Mouse and Anti-Rabbit peroxidase conjugates supplied in stabilized, liquid form
- Detection Method: Colorimetric Western blotting
- Stability: 1 year from date of receipt
- Storage: 2 - 8oC