PhosphaGLO™ AP Substrate

Size Material # List Price
30 mL 5430-0054 Contact Us
100 mL 5430-0055 Contact Us


PhosphaGLO AP Substrate is a chemiluminescent substrate for easy, reliable detection of picogram protein levels via alkaline-phosphatase (AP) in western blotting or ELISA. It contains a dioxetane-based chemiluminescent substrate designed for use with alkaline phosphatase-labeled reporter molecules. The substrate requires no special blockers.

Product is a ready-to-use, 1-component substrate requiring no special blocking solutions. Includes long glow times up to five days and allows for repeated exposures (western blot) or luminosity readings (ELISA). Easy-to-follow protocol and interchangeable with other AP chemiluminescent substrates. Product is stable for two years at 4° C. Compatible with film or with a chemiluminescent imager.

Product Highlights:

  • Offers sensitivity in the picogram range
  • Enables rapid detection on either PVDF or nitrocellulose membrane
  • Produces a sustained signal for multiple exposures


  • Detection Method: Chemiluminescent Western or dot blotting
  • Stability: 2 years from date of receipt
  • Storage: 2 - 8oC