FirePhos™ Membrane Phosphatase Substrate

Size Material # List Price
100 mL 5420-0040 Contact Us
400 mL 5420-0044 Contact Us


FirePhos Membrane Phosphatase Substrate is ideal for immunoblotting and dot blot procedures. The system is based on the hydrolysis of 5-bromo,4-chloro,3 indolylphosphate BCIP/iodonitrotetrazolium (INT) substrate by alkaline phosphatase, resulting in a strong red reaction product with minimal background staining.

It provides sensitivity in the low nanogram range, sharp band resolution and a clear image. The color produced is linear over a wide dynamic range and resists fading when exposed to light. Product is stable for a minimum of 18 months from the date of manufacture. It is supplied as a convenient, one-component solution ready for use.  Store at 2-8°C.

Product Highlights:

  • Sensitive red AP substrate ideal for multiplexing
  • Sharp band resolution and minimal background
  • Reaction linear over a wide dynamic range


  • Detection Method: Colorimetric Western or dot blotting
  • Stability: 18 months from date of manufacture.
  • Storage: 2 - 8oC