BCIP/NBT 1-Component Phosphatase Substrate

Size Material # List Price
600 mL 5420-0033 Contact Us
1 L 5420-0036 Contact Us
5 L 5420-0037 Contact Us
100 mL 5420-0038 Contact Us


BCIP/NBT Phosphatase Substrate detects proteins that have been immobilized on membranes following Western blot transfer or directly via dot blotting. This ready-to-use system is based on the hydrolysis of 5-bromo, 4-chloro, 3-indolylphosphate (BCIP) and nitroblue tetrazolium (NBT) by alkaline phosphatase, resulting in a strong purple reaction product and sensitivity in the low nanogram range. The color is stable and resists fading when exposed to light. Recommended for blotting and ELIspot Assays. 1-Component format of BCIP/NBT (5-bromo-4-chloro-3-indoyl-phosphate/nitroblue tetrazolium), no reagent preparation, hence, more convenient.

Product Highlights:

  • Most sensitive colorimetric AP substrate for blotting
  • Sharp band resolution and minimal background
  • Linear reaction over a wide dynamic range


  • Detection Method: Colorimetric Western or dot blotting
  • Stability: 1 year from date of receipt
  • Storage: 2 - 8oC