5820-0001

24-01-01

HisDetector™ Nickel-HRP

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  • Size

    0.1 mL

  • List Price

    Contact Us

  • Unit

    EA

  • Inventory Status

    In Stock

Description

HisDetector Nickel-HRP offers a fast, sensitive, single-step method for detecting recombinant His-tagged proteins. His-tagged proteins have a high affinity to nickel (Ni2+) ions. Nickel-NTA (nitrilotriacetic acid) conjugated to horseradish peroxidase (HRP) will therefore bind directly to the His-tag, eliminating the need for primary and secondary antibodies. This results in assay simplification and reduced detection times.  

Nickel-HRP offers excellent sensitivity and low background in applications such as western blotting. Product may also be used in other application (ELISA, immunohistochemistry, etc.) where direct detection of His-tagged proteins is required. Nickel-HRP is optimized for use with both chemiluminescent and colorimetric substrates.