Membrane Hybridization Buffer
Membrane Hybridization Buffer is an aqueous solution for use in the prehybridization and hybridization of biotinylated DNA probes to nucleic acids fixed on a membrane. The buffer blocks sites on the membrane to prevent nonspecific binding of probe and facilitates binding of labeled probe to homologous DNA on a membrane. Membrane Hybridization Buffer may be used in southern blots, dot blots, and colony and plaque lifts when formamide based buffers are not desirable. It is not recommended for use in northern blots.