Detector™ Random Primer DNA Biotinylation Kit
The Detector Random Primer DNA Biotinylation Kit provides a method for biotinylating DNA probes through incorporation of biotin-dCTP during random primer extension. Large quantities of biotinylated probes can be generated from a small amount of template. A template with a minimum size of 300 base pairs is required. Probe is generated in as little as 1 hour. Efficient reaction requires only 100 ng template DNA per reaction.
DNA probes are stable for up to 1 year without loss of activity. Quantitation standard allows for determination of probe specific activity. Probes labeled with biotin using the random primer method can be used on southern blots, northern blots, dot blots, in situ hybridization, colony lifts, and plaque hybridizations