SureBlue™ TMB 1-Component Microwell Peroxidase Substrate

Size Material # List Price
100 mL 5120-0075 Contact Us
400 mL 5120-0076 Contact Us
1 L 5120-0077 Contact Us
5 L 5120-0078 Contact Us


SureBlue TMB Microwell Substrate develops a deep blue color in the presence of peroxidase-labeled conjugates measurable at 650 nm and turns yellow when stopped by acidification and read at 450 nm.

Recommended for ELISA and blotting applications with addition of membrane enhancer. Single component, ready to use format of 3,3', 5, 5' - Tetramethylbenzidine exhibits exceptional stability and lot-to-lot consistency. No reagent preparation required, very convenient.

Product Highlights

  • Highly sensitive colorimetric substrate for peroxidase detection
  • Convenient, ready-to-use solution
  • Proprietary formula exhibits exceptional stability and lot-to-lot consistency.


  • Detection Method: Colorimetric ELISA
  • Sensitivity: High picogram
  • Required Enzyme: HRP
  • Measurement: After stopping with TMB BlueSTOP Solution, 620 - 650 nm; after stopping with TMB Stop Solution, 450 nm
  • Stability: 24 months from date of manufacture
  • Storage: 2 - 8oC

Lot-to-Lot Consistency of SureBlue TMB 


For each lot of SureBlue TMB, triplicate samples of HRP Anti-Human IgG Antibody were tested at concentrations ranging from 20-400 pg/well. The reaction was stopped with TMB Stop Solution, and the average optical density of the triplicate samples reported. The variation in activity among each lot of SureBlue TMB was less than 15%.