5310-0327

01-95-90-MG

BacTrace® Anti-E.coli O157:H7, Molecular Grade

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  • Size

    1.0 mg

  • List Price

    Contact Us

  • Unit

    EA

  • Inventory Status

    In Stock

Description

Affinity purified polyclonal antibody to E. coli O157:H7 Molecular Grade is isolated from a serum pool of goats immunized with heat-killed whole cells of E. coli serotype O157:H7. Molecular grade antibodies are specially processed and QC-tested to ensure that contaminating E.coli DNA is not detectable by real-time PCR. These antibodies are useful for immuno-enrichment of bacteria prior to testing by PCR or other molecular methods. 

Product Highlights:

  • High sensitivity - polyclonal antibody recognizes multiple epitopes vs. single-epitope recognition of monoclonals
  • High specificity - provides serotype-specific detection of E. coli O157:H7
  • Ideal for use in applications requiring downstream nucleic acid amplification

Specifications:

  • Immunogen: Heat-killed whole cells of E. coli serotype O157:H7
  • Purification: Affinity purification
  • Specificity: E. coli O157:H7
  • Host: Goat
  • Label: None
  • Physical State: Lyophilized
  • Stabilizers/Preservatives: None
  • Form: Whole IgG
  • Suggested Working Dilutions: Capture Antibody: 10 µg/mL, Primary Antibody: 10 μg/mL
  • Stability: 1 year from date of receipt
  • Storage: 2 - 8°C


Specificity of BacTrace Anti-E.coli Antibody

Colorimetric ELISA showing reactivity of Anti-E.coli O157:H7 Antibody with biotin/peroxidase-streptavidin detected with ABTS substrate.
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