5310-0333

01-95-96

BacTrace® Anti-E. coli O45 Species Antibody

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  • Size

    1.0 mg

  • List Price

    Contact Us

  • Unit

    EA

  • Inventory Status

    In Stock

Description

BacTrace® affinity purified polyclonal antibody to E. coli O45 is isolated from a serum pool of goats immunized with heat-killed whole cells of E. coli serogroup O45. Certain strains of E. coli serogroup O45 are considered to be STECs (shiga toxin-producing E. coli). This antibody can be used in a variety of immunoselection and immunodetection applications.

Product Highlights:

  • High specificity to E. coli O45, minimal cross-reactivity to other E. coli serogroups 
  • Polyclonal antibody yields higher sensitivity than monoclonal antibodies.
  • Standardized lots provide consistent performance.

Specifications:

  • Immunogen: Heat-killed whole cells of E. coli serotype O45
  • Purification: Affinity purification
  • Specificity: E. coli O45
  • Host: Goat
  • Form: Whole IgG
  • Label: None
  • Physical State: Lyophilized
  • Stabilizers/Preservatives: None
  • Applications: Capture Antibody: 10 μg/mL, Primary Antibody: 1 – 2 μg/mL
  • Stability: 1 year
  • Storage: 2 - 8oC



The plot depicts OD values from a TMB-detected HRP capture ELISA. SeraCare's KPL heat-killed positive controls from various STEC serogroups and S. typhimurium (1:100 dilution, ~107 CFU/mL) were used as analytes. Unlabeled anti-E. coli O45 antibody was used as the capture antibody, and HRP-labeled conjugate was used as the reporter. All other assay components were KPL catalog products. This study demonstrates the high specificity of BacTrace Anti-E.coli O45 in an ELISA. Cross reactivity is minimal with other E. coli groups and non-E. coli bacteria.