The most sensitive chromogenic substrate for peroxidase detection. It develops a deep blue color at the reaction site in the presence of peroxidase-labeled conjugates. Recommended for membrane assays and dot blots. One-component format of 3, 3', 5, 5' - tetramethylbenzidine, no reagent preparation, hence, very convenient.
Comparison of HisDetector Nickel-NTA Enzyme Conjugates with Other Methods for the Detection of His-Tagged Fusion Proteins in Western Blots