Lab Quality Control

Seraseq® gDNA TMB Mix Score 7

Material Number 0710-1326
Size 2 x 500 ng
Inventory Status In Stock
Unit EA

In immuno-oncology, the goal is to enable a patient’s immune system to locate and eliminate cancerous cells. The immune system may normally protect against cancer – except when cancers manage to evade it – since immunocompromised individuals have a higher likelihood of developing cancer. Checkpoint inhibitors, which have recently improved the prognosis for a significant number of cancer patients, work by blocking inhibitory molecules such as PD-L1 and CTLA-4 that may be allowing cancers to evade the adaptive immune system. However, by taking the brakes off the immune system, the use of checkpoint inhibitors is associated with a significant risk of developing autoimmune disease. Additionally, frequently checkpoint inhibitors are not effective.

In order to maximize the safety and efficacy of checkpoint inhibitors, it is beneficial to identify patients who are likely to respond to their use. Since the adaptive immune system can detect changes to proteins, it is thought that the number of somatic mutations that lead to such changes may be correlated with efficacy. The metric of non-silent somatic mutations per megabase of coding DNA has been termed Tumor Mutational Burden (TMB), which has shown some correlation to the efficacy of checkpoint inhibitors. Assessments of TMB are being added to many NGS assays to enable their use for patient selection in I-O clinical trials, and perhaps as companion diagnostics to these therapies. However, it is also known that TMB scores can differ significantly between assays and especially around levels that may be clinical decision points.

To improve and harmonize the measurement of TMB, we have generated and characterized tumor-normal human cell lines with different TMB scores by whole exome sequencing for use in validating targeted NGS panel-based TMB assays, and as controls for TMB measurements in I-O clinical trial projects.

The TMB reference materials are formated as genomic DNA (purified mix) and/or FFPE (requires extraction).

gDNA TMB Reference Standards:
Reference standards are made from human lung cancer cell lines and their matched “normal” cell lines from peripheral blood of the same patients.

  • 100% tumor-normal matched reference standards
  • purified gDNA mix, ready as input into WES or targeted NGS library preparation
  • range of TMB scores
  • manufactured in GMP-compliant and ISO 13485 certified facilities

Seraseq gDNA TMB Mix Non-Requirement Letter

Doc type
Safety Data Sheet (Non-Requirement Letter)

Seraseq gDNA TMB Mix Products

Doc type
Package Insert

Seraseq gDNA TMB Mix Score 7

Doc type
Product Report
Lot #

Seraseq gDNA TMB Mix

Doc type
Data Sheet

Seraseq gDNA TMB Mix Score 7

Doc type
Technical Product Report
Lot #

Seraseq gDNA TMB Non-Requirement Letter

Doc type
Safety Data Sheet (Non-Requirement Letter)

Seraseq gDNA, ctDNA and FFPE TMB Product Sheet

Doc type
Product Sheet
Product Specifications - gDNA TMB Score 7
TMB Score 7.2 ± 0.2
Fill Volume 10 µl
Concentration 50 ng/µl
Total Mass 2 x 500 ng

Immuno-oncology FAQs

Review the common questions we receive from our customers and the responses we provide.