IVD Assay Development

Seraseq® gDNA BRCA1/2 LGR Somatic Mutation Mix

Material Number 0730-0567
Size 1 x 25 μL
Inventory Status In Stock
Unit EA
Genetic testing of the tumor suppressor genes BRCA1 and BRCA2 allows for the identification of DNA variants which are associated with a significantly elevated lifetime risk of breast, ovarian, pancreatic and prostate cancer. Large genomic rearrangements (LGRs) include deletions, duplications and/or insertions often involving whole exons. Usually pathogenic, they have been reported to account for up to 27% of the overall BRCA1[1] and 5% of BRCA2 disease-causing mutations [2] with a strong founder effect accounting for about 1/3rd of all disease diagnosis. Accurate detection of a BRCA1 or BRCA2 pathogenic variant has immense impact on clinical management of disease including patient’s eligibility for new PARP inhibitors.

LGC Clinical Diagnostics has developed BRCA Reference Materials which include 10 variants in BRCA1 and 10 variants in BRCA2 ranging in size from SNVs to insertions and/or deletions over 500 bp (11 exon-level large rearrangements), resulting in a variety of phenotypic alterations at the amino acid level, including missense, nonsense, frameshift, stop-gain/loss, splice-site, and insertion/deletion of partial or up to two exons (see Product Sheet MKT-00818 for more details).

FEATURES AND BENEFITS•Develop, monitor, validate and challenge your hybrid capture-based targeted NGS assays with confidence using a highly multiplexed reference material containing biomarkers important in BRCA-driven cancers.• Single sample with 20 pathogenic BRCA1/2 variants (11 LGRs, 9 deletions, 5 SNVs, 4 indels, and 2 insertions).• Mutation targets quantitated by highly sensitive dPCR assays, and orthogonally analyzed by NGS.• All mutations are blended against the well-characterized GM24385 human genomic DNA as ‘wildtype’ background material. • Available as full process (FFPE curl) or purified genomic DNA at various allele frequencies suited for either somatic or germline testing.•Manufactured in GMP-compliant and ISO 13485-certified facilities.

Seraseq gDNA BRCA12 LGR Somatic Mutation Mix

Doc type
Product Sheet

Seraseq gDNA BRCA1/2 LGR Somatic Mutation Mix

Doc type
Technical Product Report
Lot #
10652942

Seraseq BRCA 1/2 LGR Reference Materials

Doc type
Technical Spreadsheet

Seraseq gDNA BRCA1/2 LGR Somatic Mutation Mix PI

Doc type
Package Insert

Seraseq gDNA BRCA1 Non-Requirement Letter

Doc type
Safety Data Sheet (Non-Requirement Letter)

Seraseq gDNA BRCA1/2 LGR Somatic Mutation Mix

Doc type
Safety Data Sheet (Non-Requirement Letter)

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