Controls & Reference Materials
Seraseq® HIV-1 Drug Resistance and Tropism Reference Material v2 (MF1-20)
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Specifications
HIV-1 drug resistance has tremendous impact on antiretroviral treatment success and overall health care costs. For clinical genomics assays assessing HIV-1 drug resistance, robust reference materials are critical to ensure the accurate detection of relevant mutations as low as 1%.
The ideal reference material should include a wide range of clinically relevant drug resistance mutations, mimic true viral genome complexity, assess full process variability, and be non-infectious for safe handling. Seraseq HIV-1 Drug Resistance and Tropism Reference Material v2, based on SeraCare’s patent-pending AccuPlex recombinant virus technology, meets these requirements with an innovative product.
Key features include:
- 49 clinically relevant mutations in a single sample to expedite new assay development or validation
- Variants cover Gag, Protease (PRO), Reverse Transcriptase (RT) and Integrase (INT) regions of HIV-1 genome
- Assess assay performance across entire workflow from extraction through analysis with fully extractable material in mammalian RNA viral protein coat
- Target mutation frequency of 40% to confidently assess Sanger sequencing assays
- Product is non-infectious and designed with non-replicative recombinant RNA virus
- Target concentration of 50,000 copies/mL in defibrinated human plasma
For research use only. Not for use in diagnostic procedures.
Seraseq HIV-1 Drug Resistance and Tropism Reference Material v2 (MF1-20)
Seraseq HIV-1 Drug Resistance and Tropism Reference Material Kit v2 (MF1 20)
Mutations present in the Seraseq Drug Resistance and Tropism v2 Reference Material:
| Gag | Protease Gene | RT Gene (NRTI) | RT Gene (NNRTI) | Integrase Gene |
| H219Q (CAT to CAA) | L24I (TTA to ATA) | M41L (ATG to TTG) | L100I (TA to ATA) | T661 (ACA to ATA) |
| G381S (GGC to AGC) | D30N (GAT to AAT) | K65R (AAA to AGA) | K101E (AAA to GAA) | E92Q (GAA to CAA) |
| V32I (GA to ATA) | D67N (GAC to AAC) | K103N (AAA to AAC) | E92Q (GAA to CAA) | |
| M46I (ATG to ATA) | T63S (ACT to TCT) | V108I (GTA to ATA) | T97A (ACA to GCA) | |
| I47V (ATA to CTA) | K70R (AAA to AGA) | V106A (GTA to GCA) | E138A (GAA to GCA) | |
| G48V (GGG to GTG) | L74V (TTA to GTA) | Y181C (TAT to TGT) | G140S (GGA to TCA) | |
| I50V (ATT to GTT) | F77L (TTC to CTC) | Y188L (TAT to TTA) | Y143R (TAC to CGC) | |
| I54M (ATC to ATG) | Y115F (TAT to TTT) | G190A (GGA to GCA) | Q148H (CAA to CAC) | |
| G73S (GGT to GCT) | F116Y (TTT to TAT) | P225H (CCT to CAT) | N155H (AAT to CAT) | |
| L76V (TTA to GTA) | Q151M (CAG to ATG) | M230L (ATG to CTG) | ||
| V82A (GTC to GCC) | M184V (ATG to GTG) | |||
| I84V (ATA to GTA) | l210w (TTG to TGG) | |||
| N88D (AAT to GAT) | T215Y (ACC to TAC) | |||
| L90M (TTG to ATG) | K219Q (AAA to CAA) |
For research use only. Not for use in diagnostic procedures.