Protein Detector Western Blot Kits are designed for the colorimetric detection of proteins immobilized on membranes through either electrophoresis or dot blotting. Using the Protein Detector TMB Western Blot Kit, the combination of a highly specific, stable liquid conjugate with a sensitive chromogenic substrate allows rapid and accurate identification of samples. All solutions required for blocking and washing the membrane and for diluting antibodies are provided.
For added flexibility, affinity-purified anti-mouse and anti-rabbit secondary antibodies conjugated with horseradish peroxidase are provided in stabilized, liquid form for quick dilution. TMB Membrane Peroxidase Substrate is provided as a convenient ready-to-use solution, which forms an insoluble blue precipitate when combined with the HRP-labeled antibody.
A Comparison of Blocking Solutions Used in Western Blotting