The Protein Detector LumiGLO Western Blot Kit is designed for low-picogram detection of proteins captured on immunoblots. The combination of a highly specific, stable liquid conjugate and a sensitive, chemiluminescent substrate allows rapid and accurate identification of samples. All solutions required for blocking and washing the membrane and for diluting antibodies are provided.
For added flexibility, affinity-purified anti-mouse and anti-rabbit secondary antibodies conjugated with horseradish peroxidase are provided in stabilized, liquid form for quick dilution. LumiGLO Chemiluminescent Substrate is provided as a stable two-component solution, which emits light when reacted with the HRP-labeled antibody.
Five Fast Fixes for Chemiluminescent Western Blot Disasters