KPL Immunoassay Reagents & Kits

Protein Detector™ BCIP/NBT Western Blotting Kit

Material Number 5410-0013
Size 25 blots
Inventory Status In Stock
Unit EA

The Protein Detector BCIP/NBT Western Blotting Kit is designed for the colorimetric detection of proteins immobilized on membranes through either electrophoresis or dot blotting. The combination of highly specific mouse and rabbit secondary antibody-alkaline phosphatase conjugates with a sensitive chromogenic substrate allows rapid and accurate identification of samples. All solutions required for blocking and washing the membrane and for diluting antibodies are provided.

For added flexibility, affinity-purified anti-mouse and anti-rabbit secondary antibodies conjugated with alkaline phosphatase are provided in stabilized, liquid form for quick dilution. The BCIP/NBT substrate is provided as a convenient, ready-to-use solution which forms an insoluble purple precipitate when reacted with the AP-labeled antibody. The kit is ideal for routine colorimetric detection of overexpressed protein; sensitivity level to 500 pg.

KPL Protein Detector Western Blot Kit (BCIP/NBT System)

Doc type
Certificate of Analysis
Lot #
10321221

A Comparison of Blocking Solutions Used in Western Blotting

Doc type
Application Note

KPL Protein Detector Western Blot Kit (BCIP/NBT System)

Doc type
Certificate of Analysis
Lot #
10319785

KPL Protein Detector BCIP/NBT Western Blotting Kit

Doc type
Safety Data Sheet
Lot #
10252