Metal-enhanced DAB technology permits detection of extremely low concentrations of tissue antigens. This kit provides a sensitive means for detecting peroxidase labeled antibodies and conjugates. KPL HistoMark Orange contains nickel-enhanced DAB that produces an orange-red DAB polymer and also exceeds the sensitivity of DAB alone.
The kit contains the nuclear counterstain, KPL Contrast GREEN, a solution of methyl green that contrasts sharply with orange. Blocking solution is provided to prevent endogenous peroxidase activity. The precipitates from KPL HistoMark Orange are alcohol and xylene insoluble, allowing the section to be mounted in aqueous or xylene-based mounting media. Kit contents include KPLEnhance Orange Buffer, KPL DAB-C Solution, KPL Peroxide Solution, KPL Blocking Solution Concentrate, and KPL Contrast GREEN Solution.