KPL HisDetector Western Blot Kit, AP Colorimetric uses KPL Nickel-AP and KPL BCIP/NBT 1-component substrate to produce a deep purple band at the site of reaction. Kit utilizes the specificity and high affinity of Nickel Conjugates and optimized reagents to provide picogram level sensitivity with low background. Sufficient reagents provided for running 40 blots (10 x 10 cm2 blots).
Reference number: 25-00-01.
Comparison of HisDetector Nickel-NTA Enzyme Conjugates with Other Methods for the Detection of His-Tagged Fusion Proteins in Western Blots