KPL Immunoassay Reagents & Kits

HisDetector™ Nickel-AP

Material Number 5820-0003
Size 1 mL
Inventory Status Backorder: Available Soon
Unit EA

KPL HisDetector Nickel-AP offers a fast, sensitive, single-step method for detecting recombinant His-tagged proteins. His-tagged proteins have a high affinity to nickel (Ni2+) ions. Nickel-NTA (nitrilotriacetic acid) conjugated to alkaline phosphatase(AP) will, therefore, bind directly to the His-tag, eliminating the need for primary and secondary antibodies. This results in assay simplification and reduced detection times. KPL Nickel-AP offers excellent sensitivity and low background in applications such as western blotting.

Product may also be used in other applications (ELISA, immunohistochemistry, etc.) where direct detection of His-tagged proteins is required. KPL Nickel-AP is optimized for use with both chemiluminescent and colorimetric substrates.

Reference number: 25-01-01.

KPL HisDetector Nickel-AP

Doc type
Safety Data Sheet
Lot #
10240

KPL HisDetector Nickel Conjugates

Doc type
Package Insert

Comparison of HisDetector Nickel-NTA Enzyme Conjugates with Other Methods for the Detection of His-Tagged Fusion Proteins in Western Blots

Doc type
Application Note

KPL KPL HisDetector Nickel-AP

Doc type
Certificate of Analysis
Lot #
10311338