KPL HisDetector Nickel-AP offers a fast, sensitive, single-step method for detecting recombinant His-tagged proteins. His-tagged proteins have a high affinity to nickel (Ni2+) ions. Nickel-NTA (nitrilotriacetic acid) conjugated to alkaline phosphatase(AP) will, therefore, bind directly to the His-tag, eliminating the need for primary and secondary antibodies. This results in assay simplification and reduced detection times. KPL Nickel-AP offers excellent sensitivity and low background in applications such as western blotting.
Product may also be used in other applications (ELISA, immunohistochemistry, etc.) where direct detection of His-tagged proteins is required. KPL Nickel-AP is optimized for use with both chemiluminescent and colorimetric substrates.