KPL Immunoassay Reagents & Kits

Detector™ PCR DNA Biotinylation Kit

Material Number 5910-0031
Size 30 reactions
Inventory Status Backorder: Available Soon
Unit EA

This kit provides a method for biotinylating DNA probes through incorporation of biotin-dCTP via a thermostable DNA polymerase and specific primers in the polymerase chain reaction (PCR). Large quantities of biotinylated probes can be generated from a small amount of template. A template ranging in size from 100 bp to 1.1 kbp is required. Reaction requires only 10 pg (plasmid) or 1 ng (genomic) template DNA per reaction. DNA probes are stable for up to 1 year without loss of activity.

Quantitation standard allows for determination of probe specific activity. Biotinylated PCR probes can be detected using enzyme-labeled streptavidin and a chemiluminescent or chromogenic substrate in southern blots, northern blots, dot blots, in situ hybridization, colony lifts, and plaque hybridizations.

Reference number: 60-01-01.

KPL Detector PCR DNA Biotinylation Kit

Doc type
Certificate of Analysis
Lot #

KPL Detector PCR DNA Biotinylation Kit

Doc type
Package Insert

Hybridization Buffers I Comparison of Formamide vs. Aqueous Hybridization Solutions

Doc type
Application Note