The KPL Detector HRP Chemiluminescent Kit is designed for non-radioactive detection of membrane-bound DNA using biotinylated DNA probes, peroxidase-linked streptavidin, and chemiluminescent substrate. The rapid detection procedure produces results in about 1.5 hours.
The kit contains all reagents necessary to perform hybridization and detection of membrane-bound DNA, except for the biotinylated probe. Applications include southern blotting, bacterial colony hybridizations, plaque hybridizations, and dot blotting. Detects 0.3 pg DNA after just 15 minutes with minimal background; hence optimal signal to noise ratios and blots can be stripped and reprobed.
Reference number: 54-30-00.
Hybridization Buffers I Comparison of Formamide vs. Aqueous Hybridization Solutions