A good, all-purpose substrate that offers the widest working range. Ideal when low background is required and moderate sensitivity is acceptable. ABTS develops a blue-green color in the presence of peroxidase-labeled conjugates measurable between 405 and 410 nm. No change in color when reaction is stopped. Recommended for ELISA applications.
Single component format that contains 2, 2'-azino-di(3-ethylbenzthiazoline-6-sulfonate), no reagent preparation required, hence, very convenient.