KPL Detector Block is recommended as a general blocking agent for use in membrane-based detection assays. It is especially useful when traditional blocking solutions (i.e., milk, BSA, casein) reduce sensitivity or do not adequately block background. KPL Detector Block can be used as both a blocking solution and a conjugate diluent in membrane applications such as western, northern, and southern blotting.
Reference number: 71-83-00.
Comparison of HisDetector Nickel-NTA Enzyme Conjugates with Other Methods for the Detection of His-Tagged Fusion Proteins in Western Blots