Western Blotting

Membrane-based detection assays such as western blotting and dot blotting applications offer versatile and powerful methods for characterization and analysis of proteins. SeraCare provides a complete line of KPL antibodies, conjugates, reagents, and kits for various western and dot blot applications.  

  • Expansive portfolio of SignaLOCK HRP ChemiWestern Blot Kits and universal Protein Detector Western Blot Kits, offers a broad selection of chemiluminescent and colorimetric detection systems for all user levels
  • Stable, liquid colorimetric and chemiluminescent western blotting substrates for peroxidase and phosphatase protein detection, provide a variety of performance and cost options
  • Broad selection of more than 500 KPL antibodies and conjugates to over 20 animal species' immunoglobulins; 
  • Immunoassay support reagents include conjugate stablizers, coating solutions, wash solutions, blockers and diluents, stop solutions, and buffers, reduces assay variables and improves results


View our brochure for a complete offering for western blotting reagents. For a comparison of blocking solutions in western blot analysis, review our application note

Product List

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Material # Reference # Product Name Size List Price Unit

5410-0008

54-11-50

Protein Detector™ TMB Western Blotting Kit 25 blots Contact Us EA

5410-0009

54-12-50

Protein Detector™ LumiGLO® Western Blotting Kit 25 blots Contact Us EA

5410-0010

54-53-00

SignaLOCK™ HRP ChemiWestern Kit (Film) 10 blots Contact Us EA

5410-0011

54-54-00

SignaLOCK™ HRP ChemiWestern Kit (Imager) 10 blots Contact Us EA

5410-0012

54-56-00

SignaLOCK™ AP ChemiWestern Kit (Film/Imager) 10 blots Contact Us EA

5410-0013

55-11-50

Protein Detector™ BCIP/NBT Western Blotting Kit 25 blots Contact Us EA

5420-0020

50-73-00

4CN 2-Component Membrane Peroxidase Substrate Kit 600 mL Contact Us EA

5420-0024

50-73-04

4CN 2-Component Membrane Peroxidase Substrate Kit 2.7 L Contact Us EA

5420-0025

50-77-00

TMB Membrane Peroxidase Substrate System 440 mL Contact Us EA

5420-0026

50-77-01

TMB Membrane Enhancer 40 mL Contact Us EA

5420-0027

50-77-03

TMB 1-Component Membrane Peroxidase Substrate 200 mL Contact Us EA

5420-0028

50-77-04

TMB 1-Component Membrane Peroxidase Substrate 1 L Contact Us EA

5420-0029

50-77-18

TMB 1-Component Membrane Peroxidase Substrate 100 mL Contact Us EA

5420-0030

50-81-00

BCIP/NBT Phosphatase Substrate System 300 mL Contact Us EA

5420-0033

50-81-07

BCIP/NBT 1-Component Phosphatase Substrate 600 mL Contact Us EA

5420-0036

50-81-10

BCIP/NBT 1-Component Phosphatase Substrate 1 L Contact Us EA

5420-0037

50-81-11

BCIP/NBT 1-Component Phosphatase Substrate 5 L Contact Us EA

5420-0038

50-81-18

BCIP/NBT 1-Component Phosphatase Substrate 100 mL Contact Us EA

5420-0040

50-81-30

FirePhos™ Membrane Phosphatase Substrate 100 mL Contact Us EA

5420-0044

50-81-40

FirePhos™ Membrane Phosphatase Substrate 400 mL Contact Us EA

5430-0039

54-51-00

LumiGLO® Ultra Chemiluminescent Substrate 50 mL Contact Us EA

5430-0040

54-61-00

LumiGLO® Peroxidase Chemiluminescent Substrate Kit 240 mL Contact Us EA

5430-0041

54-61-01

LumiGLO® Peroxidase Chemiluminescent Substrate Kit 720 mL Contact Us EA

5430-0042

54-61-02

LumiGLO® Peroxidase Chemiluminescent Substrate Kit 60 mL Contact Us EA

5430-0049

54-71-00

LumiGLO Reserve® Chemiluminescent Substrate Kit 2400 cm2 Contact Us EA

5430-0050

54-71-01

LumiGLO Reserve® Chemiluminescent Substrate Kit 600 cm2 Contact Us EA

5430-0051

54-71-02

LumiGLO Reserve® Chemiluminescent Substrate Kit 50 mL Contact Us EA

5430-0052

55-60-01

PhosphaGLO™ Reserve AP Substrate 30 mL Contact Us EA

5430-0053

55-60-02

PhosphaGLO™ Reserve AP Substrate 100 mL Contact Us EA

5430-0054

55-60-03

PhosphaGLO™ AP Substrate 30 mL Contact Us EA

5430-0055

55-60-04

PhosphaGLO™ AP Substrate 100 mL Contact Us EA

5450-0012

50-22-02

Silver Enhancer Kit for Membranes 250 mL Contact Us EA