HisDetector Nickel-AP offers a fast, sensitive, single-step method for detecting recombinant His-tagged proteins. His-tagged proteins have a high affinity to nickel (Ni2+) ions. Nickel-NTA (nitrilotriacetic acid) conjugated to alkaline phosphatase(AP) will therefore bind directly to the His-tag, eliminating the need for primary and secondary antibodies. This results in assay simplification and reduced detection times.
Nickel-AP offers excellent sensitivity and low background in applications such as western blotting. Product may also be used in other applications (ELISA, immunohistochemistry, etc.) where direct detection of His-tagged proteins is required. Nickel-AP is optimized for use with both chemiluminescent and colorimetric substrates.