5310-0326

01-95-90

BacTrace® Anti-Escherichia coli O157:H7 Antibody

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  • Size

    1.0 mg

  • List Price

    Contact Us

  • Unit

    EA

  • Inventory Status

    In Stock

Description

BacTrace® Anti-Escherichia coli O157:H7 Antibody is isolated from a serum pool of goats immunized with heat-killed whole cells of E. coli serotype O157:H7. Cross-reactivity to other E. coli strains has been minimized through adsorption with antigens from non-O157:H7 strains of E. coli.

Product Highlights:

  • High specificity antibody provides serotype-specific detection of Escherichia coli (E. coli) O157:H7.
  • Polyclonal antibodies produce higher signals than monoclonals. 
  • Produced under stringent controls to ensure lot-to-lot consistency

Specifications:

  • Immunogen: Heat-killed whole cells of E. coli serotype O157:H7
  • Purification: Affinity purified
  • Specificity: E. coli O157:H7
  • Host: Goat
  • Form: Whole IgG
  • Label: Unlabeled
  • Physical State: Lyophilized
  • Stabilizers/Preservatives: None
  • Suggested Working Dilutions: Capture Antibody: 10 µg/mL, Primary Antibody: 1 – 2 μg/mL
  • Stability: 1 year from date of receipt
  • Storage: 2 - 8°C

 

Specificity of BacTrace Anti-E.coli O157:H7 Antibody to Other Bacteria


Direct ELISA showing reactivity of Anti-E.coli O157:H7 Antibody detected with KPL's ABTS substrate. This study demonstrates the high specificity of BacTrace Anti-E.coli O157 with virtually no cross-reactivity to other STEC or non-STEC bacteria. 
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Specificity of BacTrace Anti-E. coli O157:H7 to the USDA's "Big Six" STECs

 Sandwich ELISA showing reactivity of Anti-E. coli O157:H7 Antibody detected with KPL’s TMB substrate. This study demonstrates the high specificity of BacTrace Anti-E. coli O157:H7 with virtually no cross-reactivity compared to STEC serogroups and S. Typhimurium.
(View larger version)