5310-0332

01-95-95

BacTrace® Anti-E. coli O121 Species Antibody

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  • Size

    1.0 mg

  • List Price

    Contact Us

  • Unit

    EA

  • Inventory Status

    In Stock

Description

Affinity purified polyclonal antibody to E. coli O121 made in goat. Isolated from a serum pool of goats immunized with heat-killed whole cells of E.coli serogroup O121. Certain strains of the E. coli serogroup O121 are considered to be STECs (shiga-toxin producing E. coli). Each lot is tested to assure specificity and lot-to-lot consistency using SeraCare's KPL in-house ELISA assay.

Product Highlights:

  • High specificity to E. coli O121 with minimal cross-reactivity to other E. coli serogroups
  • Affinity purified polyclonal antibody yields higher sensitivity than monoclonal antibodies
  • Can be used in a variety of immunoselection and immunodetection applications

Specifications:

  • Immunogen: Heat-killed whole cells of E. coli serotype O121
  • Purification: Affinity purified
  • Specificity: E. coli O121
  • Host: Goat
  • Form: Whole IgG
  • Label: Unlabeled
  • Physical State: Lyophilized
  • Stabilizers/Preservatives: None
  • Suggested Working Dilutions:Capture Antibody: 10 μg/mL, Primary Antibody: 1 – 2 μg/mL
  • Stability: 1 year from date of receipt
  • Storage: 2 - 8°C

The figure below demonstrates the high specificity of BacTrace Anti-E.coli O121 in an ELISA. Cross-reactivity is minimal with other E. coli groups and non-E. coli bacteria.

The plot depicts OD values from a TMB-detected HRP capture ELISA. SeraCare's KPL heat-killed positive controls from various STEC serogroups and S. typhimurium (1:100 dilution, ~107 CFU/mL) were used as analytes. Unlabeled Anti-E. coli O121 antibody  was used as the capture antibody, and HRP-labeled conjugate  was used as the reporter. All other assay components were SeraCare KPL products.